Confocal Microscopy Improves 3D Microdosimetry Applied to Nanoporation Experiments Targeting Endoplasmic Reticulum
نویسندگان
چکیده
منابع مشابه
Targeting pathways to the endoplasmic reticulum membrane.
The targeting of proteins to the endoplasmic reticulum (ER) membrane is the first step in the secretory pathway through which proteins are transported to the outside of the cell, the plasma membrane, and the luminal spaces and membranes of the endomembrane system. Proteins that undergo this targeting event carry a stretch of hydrophobic amino acids, usually at the amino terminus, that constitut...
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X-box binding protein-1 (XBP-1) is an important regulator of a subset of genes during endoplasmic reticulum (ER) stress. In the current study, we analyzed endogenous XBP-1 expression and localization, with a view to determining the effects of ER stress on the developmental competency of preimplantation embryos in mice. Fluorescence staining revealed that functional XBP-1 is localized on mature ...
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Caulonemata of the moss Funaria hygrometrica were vitally stained with the fluorescent, lipophilic carbocyanine dye DiOC6(3) and examined via confocal laser scanning microscopy. Although DiOCfi(3) stained nearly all of the organdies, cortical endoplasmic reticulum (ER) could be resolved under favorable conditions and appeared as a network of irregular polygons, interspersed with lamellar cister...
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ژورنال
عنوان ژورنال: Frontiers in Bioengineering and Biotechnology
سال: 2020
ISSN: 2296-4185
DOI: 10.3389/fbioe.2020.552261